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This video demonstrates a basic chromatography experiment that can be performed readily in most chemistry labs. The lab involves separation of the 2 dyes that make up the purple color in "grape koolaid".
The extraction is done manually using a disposable syringe (that can be re-used) and a DPX-RP tip. Rubbing alcohol, which contains 70% isopropanol (IPA) is the only chemical supply needed, in addition to NaCl (table salt) and water. Here is the procedure:
Kool Aid Extraction Procedure:
1. Pipette 1 mL 70% IPA and 1 mL water (salt) into test tube 1
2. Pipette 2 mL water (salt) into test tube 2
3. Pipette 2.0 mL Kool Aid into test tube 3
4. Pipette 4 mL 17% IPA into test tube 4
5. Pipette 2 mL 70% IPA into test tube 5
DPX extraction Procedure:
1. Aspirate solution 1, mix with air (app. 7 mL), wait 10 seconds, dispense
2. Aspirate solution 2, mix with air (app. 7 mL), wait 10 seconds, dispense
3. Aspirate the Kool Aid (solution 3), mix with air (app. 7 mL), wait 30 seconds, dispense
4. Repeat step 3 if the solution is not colorless-just mix and wait about 10 seconds
5. Aspirate solution 4 slowly, mix with air (app. 5 mL), wait 10 seconds, dispense
6. Add about 0.5 to 1 mL of DI water to the top of the DPX tip using a water bottle, and dispense into the test tube 4 to collect all of the red dye
7. Aspirate solution 5, mix with air (app. 7 mL), wait 10 seconds, dispense
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